Human ST2 ELISA Kit (A1518)

Human ST2 ELISA Kit

Sandwich (quantitative)

Human ST2 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human ST2 in the samples. A monoclonal antibody specific for human ST2 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for ST2, and ST2 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of ST2 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

31.25 - 2000 pg/ml

This kit can assay both natural and recombinant human ST2.

Human

This assay has shown no significant cross-reactivity.

107% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

DER-4, DER4, FIT 1, Growth stimulation expressed, homolog of mouse growth stimulation-expressed, Il1rl1, IL33R, ILRL1_HUMAN, Interleukin 1 receptor like 1, interleukin 1 receptor related protein, Interleukin-1 receptor-like 1, Protein ST2, ST2L, ST2V, T1, T1 protein