Human SP-D ELISA Kit (A1516)

Human SP-D ELISA Kit

Sandwich (quantitative)

Human SP-D ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human SP-D in the samples. A monoclonal antibody specific for human SP-D has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for SP-D, and SP-D present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of SP-D bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

78.13 - 5000 pg/ml

This kit can assay both natural and recombinant human SP-D.

Human

This assay has shown no significant cross-reactivity.

105% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

COLEC 7, COLEC7, Collectin-7, Collectin7, Lung surfactant protein D, PSP D, PSP-D, PSP-D Surfactant protein D, PSPD, Pulmonary surfactant apoprotein, Pulmonary surfactant associated protein D, Pulmonary surfactant associated protein PSP-D, Pulmonary surfactant-associated protein D, SFTP 4, SFTP4, SFTPD, SFTPD_HUMAN, SP D, Surfactant associated protein pulmonary 4, Surfactant protein D, Surfactant pulmonary associated protein D