Human RBP4 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human RBP4 in the samples. A monoclonal antibody specific for human RBP4 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for RBP4, and RBP4 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of RBP4 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Platform

Microplate

Detection Type

Colorimetric

Sample Type

Cell culture supernatant, serum and plasma.

Sensitivity

1.05 pg/ml

Product Range

31.25 - 2000 pg/ml

Specificity

This kit can assay both natural and recombinant human RBP4.

Reactivity

Human

Cross Reactivity

This assay has shown no significant cross-reactivity.

Recovery

Storage

Store at 2 - 8°C.

Synonyms

OTTHUMP00000020114, OTTHUMP00000020115, OTTHUMP00000020116, Plasma retinol binding protein 4, Plasma retinol-binding protein, Plasma retinol-binding protein(1-176), PRBP, PRO2222, RBP, RDCCAS, RET4_HUMAN, Retinol binding protein 4, Retinol binding protein 4 interstitial, Retinol binding protein 4 plasma

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.