Human/Rat PDGF BB ELISA Kit (A1546)

Human/Rat PDGF BB ELISA Kit

Sandwich (quantitative)

Human/Rat PDGF-BB ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human/rat PDGF-BB in the samples. A monoclonal antibody specific for human/rat PDGF-BB has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for PDGF-BB, and PDGF-BB present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of PDGF-BB bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

2.13 pg/ml

This kit can assay both natural and recombinant human/rat PDGF BB.

Human, Rat

This assay has shown no significant cross-reactivity.

95% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

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