This Rantes enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-Rantes Antibody. Standards or samples are then added to the microtire plate wells and Rantes, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of Rantes present in the sample, Anti-Rantes Antibody (Biotin) is added to each well to "sandwich" the Rantes immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Rantes will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Platform

Microplate

Detection Type

Colorimetric

Sample Type

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

Sensitivity

< 3 pg/ml

Product Range

15.6-1000 pg/ml

Reactivity

Human

Recovery

Storage

Store at 2-8°C for 6 months or at -20°C for 12 months.

Synonyms

Beta chemokine RANTES, Beta chemokine RANTES precursor, C C motif chemokine 5, CCL 5, CCL5, CCL5_HUMAN, Chemokine (C C motif) ligand 5, Chemokine CC Motif Ligand 5, D17S136E, EoCP, Eosinophil chemotactic cytokine, MGC17164, RANTES(4-68), Regulated upon activation normally T expressed and presumably secreted, SCYA 5, SCYA5, SIS delta, SIS-delta, SISd, Small inducible cytokine A5, Small inducible cytokine A5 (RANTES), Small inducible cytokine subfamily A (Cys Cys) member 5, Small-inducible cytokine A5, T cell specific protein p288 , T cell specific RANTES protein, T cell-specific protein P228, T-cell-specific protein RANTES, TCP 228, TCP228

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.