Human Proprotein Convertase 9 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human PCSK9 in the samples. A monoclonal antibody specific for human PCSK9 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for PCSK9, and PCSK9 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of PCSK9 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Platform

Microplate

Detection Type

Colorimetric

Sample Type

Cell culture supernatant, serum and plasma.

Sensitivity

3.39 pg/ml

Product Range

62.50 - 4000 pg/ml

Specificity

This kit can assay both natural and recombinant human PCSK9.

Reactivity

Human

Cross Reactivity

This assay has shown no significant cross-reactivity.

Recovery

Storage

Store at 2 - 8°C.

Synonyms

Convertase subtilisin/kexin type 9 preproprotein, FH3, HCHOLA3, Hypercholesterolemia autosomal dominant 3, LDLCQ1, NARC 1, NARC-1, NARC1, Neural apoptosis regulated convertase 1, Neural apoptosis-regulated convertase 1, PC 9, PC9, PCSK 9, PCSK9, PCSK9_HUMAN, Proprotein convertase PC9, Proprotein convertase subtilisin/kexin type 9, PSEC0052, Subtilisin/kexin like protease PC9, Subtilisin/kexin-like protease PC9

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.