Human Progesterone receptor ELISA Kit (A742)

$635
Freight Charges
Lead Time
7-10 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Human Progesterone receptor ELISA Kit
Assay Type
Sandwich (quantitative)
Principle of Assay
This Progesterone receptor enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-Progesterone receptor Antibody. Standards or samples are then added to the microtire plate wells and Progesterone receptor, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of Progesterone receptor present in the sample, Anti-Progesterone receptor Antibody (HRP) is added to each well to "sandwich" the Progesterone receptor immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Progesterone receptor will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Serum, plasma, urine, cell culture supernatants, and tissue samples.
Product Range
0.3-10 ng/ml
Reactivity
Human
Recovery
Storage
Store at 2-8°C for 6 months.
Synonyms
NR3C3, Nuclear receptor subfamily 3 group C member 3, PGR, PR, PRA, PRB, PRGR_HUMAN, Progestin receptor form A, Progestin receptor form B
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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