Principle of Assay
Human PALM/Paralemmin-1 ELISA Kit employs the Sandwich Enzyme Immunoassay (ELISA) technique for the quantitative measurement of human PALM/Paralemmin-1 in human. An antibody specific for PALM/Paralemmin-1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the PALM/Paralemmin-1 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-PALM/Paralemmin-1 Antibody, which binds the captured PALM/Paralemmin-1 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and Avidin conjugated to Horseradish Peroxidase (Avidin-HRP) is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of PALM/Paralemmin-1 captured in each well. The concentration of PALM/Paralemmin-1 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
