Human Osteopontin ELISA Kit (A1497)

Human Osteopontin ELISA Kit

Sandwich (quantitative)

Human Osteopontin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human OPN in the samples. A monoclonal antibody specific for human OPN has been immobilized onto microwells. Standard or samples are pipetted into the wells, and OPN present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for OPN is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of OPN bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

125 - 8000 pg/ml

This kit can assay both natural and recombinant human OPN.

Human

This assay has shown no significant cross-reactivity.

99% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

BNSP, Bone sialoprotein 1, BSP I, BSPI, Early T lymphocyte activation 1, ETA 1, ETA1, MGC110940, Nephropontin, OPN, osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein, OSTP_HUMAN, PSEC0156, Secreted phosphoprotein 1, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), SPP 1, SPP-1, SPP1, SPP1/CALPHA1 fusion, Urinary stone protein, Uropontin