Anti-Human Nuclear Antigen Antibody [235-1] (CF® 488) (A251183)

This product is discontinued

Anti-Human Nuclear Antigen Antibody [235-1] (CF® 488) (A251183) has been discontinued and is no longer available.

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Name
Anti-Human Nuclear Antigen Antibody [235-1] (CF® 488)
Description
Mouse monoclonal [235-1] antibody to Human Nuclear Antigen (CF® 488).
Specificity
This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Applications
Flow Cytometry, IF
Dilutions
Flow Cytometry: 5 µl / million cells or 5 µl / 100 µl whole blood, IF: 1:50-1:100
Reactivity
Human, Non-Human Primates
Cross Reactivity
This antibody does not cross react with Mouse, Rat, or Chicken.
Immunogen
Nuclei of human myeloid leukemia biopsy cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
235-1
Isotype
IgG1
Light Chains
kappa
Conjugate

CF® 488

Excitation: 490nm, Emission: 515nm

Purification
Protein A/G chromatography.
Concentration
100 µg/ml
Molecular Weight
70 kDa / 80 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Store at 4-8°C. Antibody is stable for 24 months.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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