Human MMP1 ELISA Kit (A661)

$635
Freight Charges
Lead Time
7-10 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Human MMP1 ELISA Kit
Assay Type
Sandwich (quantitative)
Principle of Assay
This MMP1 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-MMP1 Antibody. Standards or samples are then added to the microtire plate wells and MMP1, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of MMP1 present in the sample, Anti-MMP1 Antibody (Biotin) is added to each well to "sandwich" the MMP1 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MMP1 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.
Sensitivity
< 10 pg/ml
Product Range
156-10,000 pg/ml
Reactivity
Human
Recovery
Storage
Store at 2-8°C for 4 months or at -20°C for 8 months.
Synonyms
27 kDa interstitial collagenase, CLG, CLGN , collagenase, fibroblast, collagenase, interstitial, Fibroblast collagenase, Interstitial collagenase , Matrix metallopeptidase 1, Matrix metallopeptidase 1 (interstitial collagenase), Matrix metalloprotease 1, Matrix Metalloproteinase 1 , Matrix metalloproteinase-1, MMP 1 , MMP-1, MMP1_HUMAN, OTTHUMP00000045866
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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