This MCP3 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-MCP3 Antibody. Standards or samples are then added to the microtire plate wells and MCP3, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of MCP3 present in the sample, Anti-MCP3 Antibody (HRP) is added to each well to "sandwich" the MCP3 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MCP3 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Platform

Microplate

Detection Type

Colorimetric

Sample Type

Serum, plasma, urine, cell culture supernatants, and tissue samples.

Product Range

15-800 pg/ml

Reactivity

Human

Recovery

Storage

Store at 2-8°C for 6 months.

Synonyms

C-C motif chemokine 7, Ccl7, CCL7_HUMAN, Chemokine CC Motif Ligand 7, Chemotactic protein 3, FIC, Intercrine/chemokine MARC, MARC, MCP 3, MCP-3, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, RANTES/sis homolog, RP23-350G1.4, SCYA6 , SCYA7 , Small inducible cytokine A7 , Small-inducible cytokine A7

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.