Mouse monoclonal [A8B5] antibody to Human Kappa Light Chain (FITC).
Specificity
This antibody reacts with kappa light chains (22.5 kDa) of immunoglobulins.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity
Human
Cross Reactivity
This antibody does not cross react with Rabbit, Hamster, Sheep, Guinea Pig or Goat.
Immunogen
Unfortunately, this information is considered to be commercially sensitive.
Host
Mouse
Clonality
Monoclonal
Clone ID
A8B5
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
B lymphocytes (CD19+) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain detected by Anti-Kappa Light Chain Antibody (A86788) and lambda light chain by Anti-Lambda Light Chain Antibody (A86783), CD19 by Anti-CD19 Antibody [LT19].
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