Human Interleukin-5 ELISA Kit (A33021)

$715
Freight Charges
Lead Time
5-8 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Human Interleukin-5 ELISA Kit
Assay Type
Sandwich (quantitative)
Principle of Assay
This IL-5 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific for IL-5 and incubated. IL-5, if present, will bind and become immobilized by the antibody pre-coated on the wells and then become “sandwiched” by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL-5 and other components of the sample. In order to quantitatively determine the amount of IL-5 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits, each having a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-5, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Serum, plasma, cell culture supernatant, and other biological fluids.
Sensitivity
< 7.5 pg/ml
Product Range
7.5-1000 pg/ml
Assay Time
3h 30m
Specificity
This sandwich ELISA can detect both natural and recombinant human IL-5.
Reactivity
Human
Cross Reactivity
This assay has shown no cross-reactivity with other cytokines tested.
Recovery
Storage
Store at 2-8°C.
Synonyms
B cell differentiation factor I, B-cell differentiation factor I, Colony stimulating factor, Colony stimulating factor eosinophil, EDF , Eosinophil differentiation factor, IL 5, IL-5, IL5, IL5_HUMAN, Interleukin 5, Interleukin 5 (colony stimulating factor eosinophil), Interleukin 5 (colony stimulating factor, eosinophil), Interleukin5, T cell replacing factor, T-cell replacing factor, TRF
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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