Human Interleukin-15 ELISA Kit (A33027)

$715
Freight Charges
Lead Time
5-8 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Human Interleukin-15 ELISA Kit
Assay Type
Sandwich (quantitative)
Principle of Assay
This IL-15 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-15. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-15 and incubated. IL-15 if present, will bind and become immobilized by the antibody pre-coated on the wells and then be “sandwiched” by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL-15 and other components of the sample. In order to quantitatively determine the amount of IL-15 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethylbenzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-15, biotinconjugated antibody, and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm.

In order to measure the concentration of IL-15 in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-15 concentration (pg/mL). The concentration of IL-15 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Serum, plasma and cell culture supernatant.
Sensitivity
< 10 pg/ml
Product Range
10-2000 pg/ml
Assay Time
3h 30m
Specificity
This sandwich ELISA can detect both natural and recombinant human IL-15.
Reactivity
Human
Cross Reactivity
This assay has shown no cross-reactivity with other cytokines tested.
Recovery
Storage
Store at 2-8°C.
Synonyms
IL 15, IL-15, IL15, IL15_HUMAN, Interleukin 15, Interleukin15, MGC9721
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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