Human Interferon gamma ELISA Kit (High Sensitivity) (A323174)

Human Interferon gamma ELISA Kit (High Sensitivity)

Human Interferon gamma ELISA Kit is a high sensitivity sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Interferon gamma in serum, plasma, and other biological fluids.

Sandwich (quantitative)

Human Interferon gamma ELISA Kit (A323174) employs the sandwich Enzyme-Linked Immunoassay technique for the quantitative measurement of human Interferon gamma in serum, plasma, and other biological fluids. An antibody specific for Interferon gamma has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Interferon gamma present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Interferon gamma Antibody, which binds the captured Interferon gamma present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Interferon gamma captured in each well. The concentration of Interferon gamma can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colormetric

Colorimetric Microplate Reader

Serum, plasma, and other biological fluids.

0.98 pg/ml

3h 30m

Human

No significant cross-reactivity or interference between human Interferon gamma and analogues was observed.

Intra-assay CV: < 10%, Inter-assay CV: < 10%

Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.

IFG , IFI, IFN gamma, IFN Immune , IFN, immune, IFN-gamma, IFNG, IFNG_HUMAN, Immune interferon, Interferon Gamma Precursor, Macrophage Activating Factor , MAF, T Cell Interferon , Type II Interferon