Human IL8 ELISA Kit (A620)

$615
Freight Charges
Lead Time
7-10 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Human IL8 ELISA Kit
Assay Type
Sandwich (quantitative)
Principle of Assay
This IL8 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-IL8 Antibody. Standards or samples are then added to the microtire plate wells and IL8, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of IL8 present in the sample, Anti-IL8 Antibody (Biotin) is added to each well to "sandwich" the IL8 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL8 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.
Sensitivity
< 1 pg/ml
Product Range
15.6-1000 pg/ml (body fluids, tissue lysates, and cell culture supernatants) or 7.8-500 pg/ml (human serum and plasma).
Reactivity
Human
Recovery
Storage
Store at 2-8°C for 6 months or at -20°C for 12 months.
Synonyms
(Ala-IL-8)77, (Ser-IL-8)72, 3-10C, 9E3, AMCF-I, B-ENAP, Beta thromboglobulin like protein, Beta-thromboglobulin-like protein, C X C motif chemokine 8, C-X-C motif chemokine 8, CEF-4, Chemokine (C X C motif) ligand 8, chemokine, CXC motif, ligand 8, CXCL8 , Emoctakin, GCP 1, GCP-1, GCP/IL-8 protein I, GCP/IL-8 protein II, GCP/IL-8 protein III, GCP/IL-8 protein IV, GCP/IL-8 protein V, GCP/IL-8 protein VI, GCP1 , Granulocyte chemotactic protein 1, IL 8, IL-8, IL-8(1-77), IL-8(9-77), IL8/NAP1 form I, IL8/NAP1 form II, IL8/NAP1 form III, IL8/NAP1 form IV, IL8/NAP1 form V, IL8/NAP1 form VI, IL8_HUMAN, Inteleukin 8, Interleukin 8, K60, LECT , LUCT , Lymphocyte derived neutrophil activating factor, Lymphocyte-derived neutrophil-activating factor, LYNAP, MDNCF, MDNCF-b, MDNCF-c, MONAP, Monocyte derived neutrophil activating peptide, Monocyte derived neutrophil chemotactic factor, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, NAF, NAP 1, NAP-1, NAP1 , Neutrophil activating peptide 1, Neutrophil activating protein 1, Neutrophil-activating factor, Neutrophil-activating protein 1, Protein 3 10C, Protein 3-10C, SCYB8, Small inducible cytokine subfamily B member 8, T cell chemotactic factor, T-cell chemotactic factor, TSG-1, TSG1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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