Human IL10 ELISA Kit (A621)

$615
Freight Charges
Lead Time
7-10 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Human IL10 ELISA Kit
Assay Type
Sandwich (quantitative)
Principle of Assay
This IL10 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-IL10 Antibody. Standards or samples are then added to the microtire plate wells and IL10, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of IL10 present in the sample, Anti-IL10 Antibody (Biotin) is added to each well to "sandwich" the IL10 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL10 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
Platform
Microplate
Detection Type
Colorimetric
Sensitivity
< 0.5 pg/ml
Product Range
7.8-500 pg/ml (body fluids, tissue lysates, and cell culture supernatants) or 3.4-250 pg/ml (human serum and plasma).
Reactivity
Human
Recovery
Storage
Store at 2-8°C for 4 months or at -20°C for 8 months.
Synonyms
CSIF, Cytokine synthesis inhibitory factor, GVHDS, IL 10, IL-10, IL10A, IL10_HUMAN, Interleukin 10 , Interleukin-10, Interleukin10, MGC126450, MGC126451, T cell growth inhibitory factor, T-cell growth inhibitory factor, TGIF
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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