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Recombinant Anti-Human IgG4 Fc Antibody [RM217] (Biotin) (A121273)

Multiplex Immunoassay - Anti-Human IgG4 Fc Antibody (Biotin) [RM217] (A121273) - Antibodies.com ELISA - Anti-Human IgG4 Fc Antibody (Biotin) [RM217] (A121273) - Antibodies.com ELISA - Anti-Human IgG4 Fc Antibody (Biotin) [RM217] (A121273) - Antibodies.com ELISA - Anti-Human IgG4 Fc Antibody (Biotin) [RM217] (A121340) - Antibodies.com
Recombinant Antibody
$480

Recombinant rabbit monoclonal [RM217] antibody to Human IgG4 Fc (Biotin) for Flow Cytometry and ELISA.

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Freight Charges
$40 to United States
Lead Time
4-6 Business Days

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+1 (314) 370-6046
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Name
Anti-Human IgG4 Fc Antibody [RM217] (Biotin)
Description
Recombinant rabbit monoclonal [RM217] antibody to Human IgG4 Fc (Biotin).
Specificity
This antibody recognizes the Fc region of human IgG4.
Applications
Flow Cytometry, ELISA
Dilutions
ELISA: 50-200 ng/well (Capture) and 0.05-0.2 µg/ml (Detection)
Reactivity
Human
Cross Reactivity
There is no cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Immunogen
Synthetic peptide corresponding to the Fc region of human IgG4.
Host
Rabbit
Clonality
Monoclonal
Clone ID
RM217
Isotype
IgG
Conjugate

Biotin

Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Multiplex Immunoassay - Anti-Human IgG4 Fc Antibody (Biotin) [RM217] (A121273) - Antibodies.comEnlarge Image
Figure 1: Anti-Human IgG4 Fc Antibody [RM217] (Biotin) (A121273)
Anti-Human IgG4 Fc Antibody [RM217] (Biotin) specifically reacts to human IgG4. There is no cross reactivity with human IgG1, IgG2, or IgG3.
ELISA - Anti-Human IgG4 Fc Antibody (Biotin) [RM217] (A121273) - Antibodies.comEnlarge Image
Figure 2: Anti-Human IgG4 Fc Antibody [RM217] (Biotin) (A121273)
Sandwich ELISA using Anti-Human IgG4 Fc Antibody [RM217] as the capture antibody, and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an AP conjugated streptavidin.
ELISA - Anti-Human IgG4 Fc Antibody (Biotin) [RM217] (A121273) - Antibodies.comEnlarge Image
Figure 3: Anti-Human IgG4 Fc Antibody [RM217] (Biotin) (A121273)
Sandwich ELISA using Anti-Human IgG4 Fc Antibody [RM217] as the capture antibody, and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an AP conjugated streptavidin.
ELISA - Anti-Human IgG4 Fc Antibody (Biotin) [RM217] (A121340) - Antibodies.comEnlarge Image
Figure 4: Anti-Human IgG4 Fc Antibody [RM217] (Biotin) (A121273)
A titer ELISA using Anti-Human IgG4 Fc Antibody [RM217]. The plate was coated with different amounts of human IgG4. A serial dilution of Anti-Human IgG4 Fc Antibody [RM217] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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