Recombinant Anti-Human IgG4 Antibody [RM120] (A121351)

Name: Anti-Human IgG4 Antibody [RM120]
Brand: Antibodies.com
SKU: A121351
Price: 480.0 USD
Availability: InStock

Enlarge Image

$480

Recombinant rabbit monoclonal [RM120] antibody to Human IgG4 for WB, ICC, IHC and Flow Cytometry.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

4-6 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Tags:

Recombinant Antibody

Specifications

Name

Anti-Human IgG4 Antibody [RM120]

Description

Recombinant rabbit monoclonal [RM120] antibody to Human IgG4.

Specificity

This antibody recognizes the heavy chain of human IgG4.

Applications

WB, ICC, IHC, Flow Cytometry

Dilutions

ELISA: 50-200 ng/well (Capture) and 0.05-0.2 µg/ml (Detection), ICC: 110 µg/ml, IHC: 110 µg/ml

Reactivity

Human

Cross Reactivity

There is no cross reactivity to human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, monkey IgG, mouse IgG, rat IgG, or goat IgG.

Immunogen

Synthetic peptide corresponding to the hinge region of human IgG4.

Host

Rabbit

Clonality

Monoclonal

Clone ID

RM120

Isotype

IgG

Conjugate

Unconjugated

Purification

Protein A chromatography.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Ig gamma 4 chain C region, IGHG4, immunoglobulin heavy chain constant region gamma 4, Immunoglobulin heavy constant gamma 4 (G4m marker), MGC117419

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

Enlarge Image

Figure 1: Anti-Human IgG4 Antibody [RM120] (A121351)

Detection of human IgG4 in monkey serum, using Anti-Human IgG4 Antibody [RM120] (capture antibody) and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) (detection antibody) as a Sandwich ELISA pair. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.

Enlarge Image

Figure 2: Anti-Human IgG4 Antibody [RM120] (A121351)

Sandwich ELISA using Anti-Human IgG4 Antibody [RM120] as the capture antibody, and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an AP conjugated streptavidin.

Enlarge Image

Figure 3: Western Blot - Anti-Human IgG4 Antibody [RM120] (A121351)

Western blot of human, mouse, rat, and goat IgG shows Anti-Human IgG4 Antibody [RM120] reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. There is no cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG.

Enlarge Image

Figure 4: Anti-Human IgG4 Antibody [RM120] (A121351)

A titer ELISA using Anti-Human IgG4 Antibody [RM120]. The plate was coated with different amounts of human IgG4. A serial dilution of Anti-Human IgG4 Antibody [RM120] was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgGas the secondary antibody.