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Recombinant Anti-Human IgG3 Antibody [RM119] (Biotin) (A121271)

Immunohistochemistry - Anti-Human IgG3 Antibody (Biotin) [RM119] (A121271) - Antibodies.com ELISA - Anti-Human IgG3 Antibody (Biotin) [RM119] (A121271) - Antibodies.com ELISA - Anti-Human IgG3 Antibody (Biotin) [RM119] (A121271) - Antibodies.com ELISA - Anti-Human IgG3 Antibody (Biotin) [RM119] (A121271) - Antibodies.com ELISA - Anti-Human IgG3 Antibody (Biotin) [RM119] (A121339) - Antibodies.com
Recombinant Antibody
$480

Recombinant rabbit monoclonal [RM119] antibody to Human IgG3 (Biotin) for ICC, IHC, Flow Cytometry and ELISA.

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Freight Charges
$40 to United States
Lead Time
4-6 Business Days

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+1 (314) 370-6046
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orders@antibodies.com

Name
Anti-Human IgG3 Antibody [RM119] (Biotin)
Description
Recombinant rabbit monoclonal [RM119] antibody to Human IgG3 (Biotin).
Specificity
This antibody recognizes the heavy chain of human IgG3.
Applications
ICC, IHC, Flow Cytometry, ELISA
Dilutions
ELISA: 50-200 ng/well (Capture) and 0.05-0.2 µg/ml (Detection), ICC: 0.5-2 µg/ml, IHC: 0.5-2 µg/ml
Reactivity
Human
Cross Reactivity
There is no cross reactivity with human IgG1, IgG2, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Immunogen
Human IgG3.
Host
Rabbit
Clonality
Monoclonal
Clone ID
RM119
Isotype
IgG
Conjugate

Biotin

Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
DKFZp686H11213, FLJ39988, FLJ40036, FLJ40253, FLJ40587, FLJ40789, FLJ40834, HDC, Heavy chain disease protein, Ig gamma 3 chain C region, IGHG3, Immunoglobulin Gm3, Immunoglobulin heavy constant gamma 3 (G3m marker), MGC45809
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immunohistochemistry - Anti-Human IgG3 Antibody (Biotin) [RM119] (A121271) - Antibodies.comEnlarge Image
Figure 1: Immunohistochemistry - Anti-Human IgG3 Antibody [RM119] (Biotin) (A121271)
Immunohistochemistry of Human Lymphoid Tissue using Anti-Human IgG3 Antibody [RM119] (Biotin).
ELISA - Anti-Human IgG3 Antibody (Biotin) [RM119] (A121271) - Antibodies.comEnlarge Image
Figure 2: Anti-Human IgG3 Antibody [RM119] (Biotin) (A121271)
Sandwich ELISA using Anti-Human IgG3 Antibody [RM119] as the capture antibody (100ng/well), and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an HRP conjugated streptavidin.
ELISA - Anti-Human IgG3 Antibody (Biotin) [RM119] (A121271) - Antibodies.comEnlarge Image
Figure 3: Anti-Human IgG3 Antibody [RM119] (Biotin) (A121271)
Sandwich ELISA using Anti-Human IgG3 Antibody [RM119] as the capture antibody (100ng/well), and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an HRP conjugated streptavidin.
ELISA - Anti-Human IgG3 Antibody (Biotin) [RM119] (A121271) - Antibodies.comEnlarge Image
Figure 4: Anti-Human IgG3 Antibody [RM119] (Biotin) (A121271)
ELISA of human immunoglobulins shows Anti-Human IgG3 Antibody [RM119] reacts only to human IgG3. No cross reactivity with Human IgG1, IgG2, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml, or 10ng/ml of Anti-Human IgG3 Antibody [RM119] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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