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Recombinant Anti-Human IgG2 Antibody [RM118] (Biotin) (A121270)

ELISA - Anti-Human IgG2 Antibody (Biotin) [RM118] (A121270) - Antibodies.com ELISA - Anti-Human IgG2 Antibody (Biotin) [RM118] (A121270) - Antibodies.com ELISA - Anti-Human IgG2 Antibody (Biotin) [RM118] (A121270) - Antibodies.com ELISA - Anti-Human IgG2 Antibody (Biotin) [RM118] (A121338) - Antibodies.com
Recombinant Antibody
$480

Recombinant rabbit monoclonal [RM118] antibody to Human IgG2 (Biotin) for Flow Cytometry and ELISA.

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Freight Charges
$40 to United States
Lead Time
4-6 Business Days

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+1 (314) 370-6046
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Name
Anti-Human IgG2 Antibody [RM118] (Biotin)
Description
Recombinant rabbit monoclonal [RM118] antibody to Human IgG2 (Biotin).
Specificity
This antibody recognizes the heavy chain of human IgG2.
Applications
Flow Cytometry, ELISA
Dilutions
ELISA: 50-200 ng/well (Capture) and 0.05-0.2 µg/ml (Detection), ICC: 0.5-2 µg/ml, IHC: 0.5-2 µg/ml
Reactivity
Human
Cross Reactivity
There is no cross reactivity with human IgG1, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Immunogen
Human IgG2.
Host
Rabbit
Clonality
Monoclonal
Clone ID
RM118
Isotype
IgG
Conjugate

Biotin

Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Ig gamma-2 chain C region, IGHG2, immunoglobulin Gm2, immunoglobulin heavy constant gamma 2 (G2m marker), immunoglobulin heavy constant, gamma 2
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
ELISA - Anti-Human IgG2 Antibody (Biotin) [RM118] (A121270) - Antibodies.comEnlarge Image
Figure 1: Anti-Human IgG2 Antibody [RM118] (Biotin) (A121270)
Sandwich ELISA using Anti-Human IgG2 Antibody [RM118] as the capture antibody, and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an AP conjugated streptavidin.
ELISA - Anti-Human IgG2 Antibody (Biotin) [RM118] (A121270) - Antibodies.comEnlarge Image
Figure 2: Anti-Human IgG2 Antibody [RM118] (Biotin) (A121270)
Sandwich ELISA using Anti-Human IgG2 Antibody [RM118] as the capture antibody, and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an AP conjugated streptavidin.
ELISA - Anti-Human IgG2 Antibody (Biotin) [RM118] (A121270) - Antibodies.comEnlarge Image
Figure 3: Anti-Human IgG2 Antibody [RM118] (Biotin) (A121270)
ELISA of human immunoglobulins shows Anti-Human IgG2 Antibody [RM118] only reacted to human IgG2. No cross reactivity with Human IgG1, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50ng/well of different immunoglobulins. A serial dilution of Anti-Human IgG2 Antibody [RM118] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA - Anti-Human IgG2 Antibody (Biotin) [RM118] (A121338) - Antibodies.comEnlarge Image
Figure 4: Anti-Human IgG2 Antibody [RM118] (Biotin) (A121270)
A titer ELISA using Anti-Human IgG2 Antibody [RM118]. The plate was coated with different amounts of human IgG2. A serial dilution of Anti-Human IgG2 Antibody [RM118] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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