Human IGFBP-3 ELISA Kit (A1449)

Human IGFBP-3 ELISA Kit

Sandwich (quantitative)

Human IGFBP-3 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human IGFBP-3 in the samples. A monoclonal antibody specific for human IGFBP-3 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for IGFBP-3, and IGFBP-3 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IGFBP-3 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

125 - 8000 pg/ml

This kit can assay both natural and recombinant human IGFBP-3.

Human

This assay has shown no significant cross-reactivity.

Store at 2 - 8°C.

This product is for research use only. It is not intended for diagnostic or therapeutic use.