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Recombinant Anti-Human IgA2 Antibody [RM125] (A121349)

Immunohistochemistry - Anti-Human IgA2 Antibody [RM125] (A121349) - Antibodies.com ELISA - Anti-Human IgA2 Antibody [RM125] (A121349) - Antibodies.com ELISA - Anti-Human IgA2 Antibody [RM125] (A121349) - Antibodies.com ELISA - Anti-Human IgA2 Antibody [RM125] (A121349) - Antibodies.com ELISA - Anti-Human IgA2 Antibody [RM125] (A121277) - Antibodies.com
Recombinant Antibody
$480

Recombinant rabbit monoclonal [RM125] antibody to Human IgA2 for ICC, IHC, Flow Cytometry and ELISA.

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Freight Charges
$40 to United States
Lead Time
4-6 Business Days

Telephone
+1 (314) 370-6046
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Name
Anti-Human IgA2 Antibody [RM125]
Description
Recombinant rabbit monoclonal [RM125] antibody to Human IgA2.
Specificity
This antibody recognizes human IgA2.
Applications
ICC, IHC, Flow Cytometry, ELISA
Dilutions
ELISA: 50-200 ng/well (Capture) and 0.05-0.2 µg/ml (Detection), ICC: 0.5-2 µg/ml, IHC: 0.1-1 µg/ml
Reactivity
Human
Cross Reactivity
There is no cross reactivity with human IgA1, IgG, IgM, IgD, or IgE.
Immunogen
Human IgA2.
Host
Rabbit
Clonality
Monoclonal
Clone ID
RM125
Isotype
IgG
Conjugate

Unconjugated

Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
A2m marker, Ig alpha 2 chain C region, IgA constant heavy chain 2, IGHA 2, IGHA2, IGHA2 protein, Immunoglobulin A2, Immunoglobulin Am2, Immunoglobulin heavy constant alpha 2, immunoglobulin heavy constant alpha 2 (A2m marker)
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immunohistochemistry - Anti-Human IgA2 Antibody [RM125] (A121349) - Antibodies.comEnlarge Image
Figure 1: Immunohistochemistry - Anti-Human IgA2 Antibody [RM125] (A121349)
Immunohistochemical staining of human tonsil tissue using Anti-Human IgA2 Antibody [RM125].
ELISA - Anti-Human IgA2 Antibody [RM125] (A121349) - Antibodies.comEnlarge Image
Figure 2: Anti-Human IgA2 Antibody [RM125] (A121349)
Sandwich ELISA using Anti-Human IgA2 Antibody [RM125] as the capture antibody (100ng/well), and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
ELISA - Anti-Human IgA2 Antibody [RM125] (A121349) - Antibodies.comEnlarge Image
Figure 3: Anti-Human IgA2 Antibody [RM125] (A121349)
Sandwich ELISA using Anti-Human IgA2 Antibody [RM125] as the capture antibody (100ng/well), and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
ELISA - Anti-Human IgA2 Antibody [RM125] (A121349) - Antibodies.comEnlarge Image
Figure 4: Anti-Human IgA2 Antibody [RM125] (A121349)
ELISA of human immunoglobulins shows Anti-Human IgA2 Antibody [RM125] reacts only to Human IgA2. There is no cross reactivity with Human IgA1, IgG, IgM, IgD, or IgE. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml, or 10ng/ml of Anti-Human IgA2 Antibody [RM125] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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