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Recombinant Anti-Human IgA1 Antibody [RM124] (Biotin) (A121277)

Immunohistochemistry - Anti-Human IgA1 Antibody (Biotin) [RM124] (A121277) - Antibodies.com ELISA - Anti-Human IgA1 Antibody (Biotin) [RM124] (A121277) - Antibodies.com ELISA - Anti-Human IgA1 Antibody (Biotin) [RM124] (A121277) - Antibodies.com ELISA - Anti-Human IgA1 Antibody (Biotin) [RM124] (A121277) - Antibodies.com ELISA - Anti-Human IgA1 Antibody (Biotin) [RM124] (A121344) - Antibodies.com
Recombinant Antibody
$495
Product Datasheet

Recombinant rabbit monoclonal [RM124] antibody to Human IgA1 (Biotin) for ICC, IHC, Flow Cytometry and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-Human IgA1 Antibody [RM124] (Biotin)
Description
Recombinant rabbit monoclonal [RM124] antibody to Human IgA1 (Biotin).
Specificity
This antibody recognizes human IgA1, and very slightly cross reacts with human IgA2.
Applications
ICC, IHC, Flow Cytometry, ELISA
Dilutions
ELISA: 50-200 ng/well (Capture) and 0.05-0.2 µg/ml (Detection), ICC: 0.5-2 µg/ml, IHC: 0.1-1 µg/ml
Reactivity
Human
Cross Reactivity
There is no cross reactivity with human IgG, IgM, IgD, or IgE.
Immunogen
Human IgA.
Host
Rabbit
Clonality
Monoclonal
Clone ID
RM124
Isotype
IgG
Conjugate

Biotin

Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (5)

Immunohistochemistry - Anti-Human IgA1 Antibody (Biotin) [RM124] (A121277) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Human IgA1 Antibody [RM124] (Biotin) (A121277)

Immunohistochemistry of Human Tonsil Tissue using Anti-Human IgA1 Antibody [RM124] (Biotin).
ELISA - Anti-Human IgA1 Antibody (Biotin) [RM124] (A121277) - Antibodies.comEnlarge Image

Anti-Human IgA1 Antibody [RM124] (Biotin) (A121277)

Sandwich ELISA using Anti-Human IgA1 Antibody [RM124] as the capture antibody (100ng/well), and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an AP conjugated streptavidin.
ELISA - Anti-Human IgA1 Antibody (Biotin) [RM124] (A121277) - Antibodies.comEnlarge Image

Anti-Human IgA1 Antibody [RM124] (Biotin) (A121277)

Sandwich ELISA using Anti-Human IgA1 Antibody [RM124] as the capture antibody (100ng/well), and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an AP conjugated streptavidin.
ELISA - Anti-Human IgA1 Antibody (Biotin) [RM124] (A121277) - Antibodies.comEnlarge Image

Anti-Human IgA1 Antibody [RM124] (Biotin) (A121277)

ELISA of human immunoglobulins shows Anti-Human IgA1 Antibody [RM124] reacts only to Human IgA. Very slightly cross reacts with IgA2. No cross reactivity with Human IgG, IgM, IgD, or IgE. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml, or 10ng/ml of Anti-Human IgA1 Antibody [RM124] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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