This HSP27 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-HSP27 Antibody. Standards or samples are then added to the microtire plate wells and HSP27, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of HSP27 present in the sample, Anti-HSP27 Antibody (Biotin) is added to each well to "sandwich" the HSP27 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HSP27 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Platform

Microplate

Detection Type

Colorimetric

Sample Type

Serum, body fluids, tissue lysates, and cell culture supernatants.

Sensitivity

< 5 pg/ml

Product Range

62.5-4000 pg/ml

Reactivity

Human

Recovery

Storage

Store at 2-8°C for 4 months or at -20°C for 8 months.

Synonyms

28 kDa heat shock protein, CMT2F, DKFZp586P1322, epididymis secretory protein Li 102, Estrogen regulated 24 kDa protein, Estrogen-regulated 24 kDa protein, Heat shock 25kDa protein 1, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27kDa protein, Heat shock 27kDa protein 1, Heat shock 28kDa protein 1, Heat Shock Protein 27, Heat shock protein beta 1, Heat shock protein beta-1, heat shock protein family B (small) member 1, HEL-S-102, HMN2B, HS.76067, Hsp 25, HSP 27, Hsp 28, Hsp B1, Hsp25, Hsp28, HspB1, HSPB1_HUMAN, SRP27, Stress responsive protein 27, Stress-responsive protein 27

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.