Human HMGN1 ELISA Kit is a sandwich (quantitative) Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human HMGN1 in tissue homogenates, cell lysates or other biological fluids.

Assay Type

Sandwich (quantitative)

Principle of Assay

Human HMGN1 ELISA Kit employs the Sandwich Enzyme Immunoassay (ELISA) technique for the quantitative measurement of human HMGN1 in human. An antibody specific for HMGN1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the HMGN1 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-HMGN1 Antibody, which binds the captured HMGN1 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and Avidin conjugated to Horseradish Peroxidase (Avidin-HRP) is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of HMGN1 captured in each well. The concentration of HMGN1 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Platform

Pre-coated Microplate (12 x 8 well strips)

Detection Type

Colourmetric

Instrument

Colorimetric Microplate Reader

Sample Type

Tissue homogenates, cell lysates or other biological fluids.

Sensitivity

0.114 ng/ml

Product Range

0.312-20 ng/ml

Assay Time

1 - 4.5 Hours

Reactivity

Human

Recovery

Sample Type	n	Range	Average
Serum	5	80% - 102%	91%
EDTA Plasma	5	81% - 100%	90%
Heparin Plasma	5	80% - 89%	84%

Linearity

Sample Type	1:2	1:4	1:8	1:16
Serum (n=5)	87-91%	87-107%	74-101%	92-97%
EDTA Plasma (n=5)	90-105%	84-101%	90-101%	79-108%
Heparin Plasma (n=5)	84-95%	92-105%	82-105%	89-91%

Precision

Intra-assay: CV < 10%, Inter-assay: CV < 12%

General Notes

Information online is representative. Always refer to the Product Manual inside the kit.

Storage

Store at +4°C. Do not use past expiration date!

Synonyms

FLJ27265, FLJ31471, High mobility group (nonhistone chromosomal) protein 14, High mobility group nucleosome binding 1, High mobility group nucleosome binding domain 1, High mobility group nucleosome binding domain containing protein 1, High mobility group nucleosome-binding domain-containing protein 1, High mobility group protein 14, HMG 14, HMG14, HMGN 1, HMGN1_HUMAN, MGC104230, MGC117425, Non-histone chromosomal protein HMG-14, Nonhistone chromosomal protein HMG 14, Nonhistone chromosomal protein HMG14

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.