Human GM-CSF ELISA Kit (A1437)

$510
Freight Charges
Lead Time
3-5 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Human GM-CSF ELISA Kit
Assay Type
Sandwich (quantitative)
Principle of Assay
Human GM-CSF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human GM-CSF in the samples. A monoclonal antibody specific for human GM-CSF has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for GM-CSF, and GM-CSF present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of GM-CSF bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
4.76 pg/ml
Product Range
7.81 - 500 pg/ml
Specificity
This kit can assay both natural and recombinant human GM-CSF.
Reactivity
Human
Cross Reactivity
This assay has shown no significant cross-reactivity.
Recovery
104% (Average Recovery from Serum Samples).
Storage
Store at 2 - 8°C.
Synonyms
Colony stimulating factor, Colony Stimulating Factor 2, Colony stimulating factor 2 (granulocyte-macrophage), Colony-stimulating factor, CSF, CSF 2, CSF2, CSF2_HUMAN, GMCSF, Granulocyte Macrophage Colony Stimulating Factor, Granulocyte-macrophage colony-stimulating factor, MGC131935, MGC138897, MGI1GM, Molgramostin, Pluripoietin-a, Sargramostim
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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