Human Fas ELISA Kit (A1428)

Human Fas ELISA Kit

Sandwich (quantitative)

Human Fas ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Fas in the samples. A monoclonal antibody specific for human Fas has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for Fas, and Fas present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Fas bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

3.15 pg/ml

This kit can assay both natural and recombinant human Fas.

Human

This assay has shown no significant cross-reactivity.

102% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

ALPS 1A, ALPS1A, APO 1, Apo 1 antigen, APO 1 cell surface antigen, Apo-1 antigen, APO1, Apo1 antigen, APO1 cell surface antigen, Apoptosis antigen 1, Apoptosis mediating surface antigen FAS, Apoptosis-mediating surface antigen FAS, APT 1, APT1, CD 95, CD 95 antigen, CD95, CD95 antigen , Delta Fas, Delta Fas/APO 1/CD95, Delta Fas/APO1/CD95, Fas (TNF receptor superfamily, member 6), FAS 1, FAS 827dupA, Fas AMA, FAS Antigen, Fas cell surface death receptor, FAS1, FASLG receptor, FASTM, sFAS, Surface antigen APO1, TNF receptor superfamily, member 6, TNFRSF 6, TNFRSF6, TNR6_HUMAN, Tumor necrosis factor receptor superfamily member 6