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Human Eph Receptor A1/EphA1 ELISA Kit (A2358)

Standard Curve - Human Eph Receptor A1/EphA1 ELISA Kit (A2358) - Antibodies.com
$585
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Name
Human Eph Receptor A1/EphA1 ELISA Kit
Description
Human Eph Receptor A1/EphA1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Eph Receptor A1/EphA1 in tissue homogenates, cell lysates or other biological fluids.
Assay Type
Sandwich (quantitative)
Principle of Assay
Human Eph Receptor A1/EphA1 ELISA Kit (A2358) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Eph Receptor A1/EphA1 in tissue homogenates, cell lysates or other biological fluids. An antibody specific for Eph Receptor A1/EphA1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Eph Receptor A1/EphA1 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Eph Receptor A1/EphA1 Antibody, which binds the captured Eph Receptor A1/EphA1 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Eph Receptor A1/EphA1 captured in each well. The concentration of Eph Receptor A1/EphA1 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colourmetric
Instrument
Colorimetric Microplate Reader
Sample Type
Tissue homogenates, cell lysates or other biological fluids.
Sensitivity
0.124 ng/ml
Product Range
0.312-20 ng/ml
Assay Time
4h 30m
Reactivity
Human
Recovery
Sample Type n Range Average
Serum 5 80% - 102% 91%
EDTA Plasma 5 81% - 100% 90%
Heparin Plasma 5 80% - 89% 84%
Linearity
Sample Type 1:2 1:4 1:8 1:16
Serum (n=5) 87-91% 87-107% 74-101% 92-97%
EDTA Plasma (n=5) 90-105% 84-101% 90-101% 79-108%
Heparin Plasma (n=5) 84-95% 92-105% 82-105% 89-91%
Precision
Intra-assay: CV < 10%, Inter-assay: CV < 12%
General Notes
Information online is representative. Always refer to the Product Manual inside the kit.
Storage
Store kit components at +4°C or -20°C as instructed. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Kit Components
Item Quantity Storage
Pre-Coated 96 Well Microplate 12 x 8 Well Strips -20°C
Lyopholized Standard 2 Vials -20°C
Detection Solution A 120μl -20°C
Detection Solution B 120µl -20°C
Wash Buffer (30X) 20ml +4°C
Sample Dilution Buffer 45ml -20°C
TMB Substrate 9ml +4°C
Stop Solution 6ml +4°C
Plate Sealers 5 Adhesive Strips -
Required Materials
The following materials are not included in the kit, but are required to run this assay:
  • Microplate reader capable of measuring absorbance at 450nm ± 10nm.
  • Squirt bottle, multichannel pipette reservoir, or automated microplate washer.
  • Graph paper or computer software capable of generating logarithmic functions.
  • Test tubes and Eppendorf tubes to prepare standards and samples.
  • Multi- and single-channel pipettes and sterile pipette tips.
  • Deionized or distilled water.
  • Absorbent paper towels.
  • 37°C incubator.
Important Notes
  • Sample concentrations should be estimated before being used in the assay and a proper dilution factor should be selected to make the diluted target protein concentration fall in the optimal detection range of this kit.
  • Hemolysis can greatly impact the validity of test results. Take care to minimize hemolysis.
  • Samples should be brought to room temperature before starting the assay.
Bring all reagents and samples to room temperature before use.
We recommend that all standards and samples be assayed in duplicate.
Step 1
Prepare all reagents, samples, and working standards as directed in the previous sections.
Step 2
Determine the number of microplate strips required for the assay and insert them into the plate frame. Unused strips should be stored in a foil pouch at -20°C.
Step 3
Add 100μl of standard solutions to the standard wells. Note: A plate layout is provided to record standards and samples assayed.
Step 4
Add 100μl of samples to the sample wells.
Step 5
Cover with an adhesive plate sealer provided and incubate at 37°C for 2 hours.
Step 6
Remove the plate sealer and aspirate the liquid from the plate.
Step 7
Add 100μl of working Detection Solution A to each well.
Step 8
Cover with a new adhesive plate sealer and incubate at 37°C for 60 minutes.
Step 9
Remove the plate sealer, aspirate the liquid from the plate, and wash the plate three times with Wash Buffer. Soak wells with 300μl of Wash Buffer for 1-2 minutes each time. Note: Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Blot the plate onto paper towels or other absorbent material. Do not let the wells dry completely at any time!
Step 10
Add 100μl of working Detection Solution B to each well.
Step 11
Cover with a new adhesive plate sealer and incubate at 37°C for 60 minutes.
Step 12
Remove the plate sealer, aspirate the liquid from the plate, and wash the plate five times with Wash Buffer. Soak wells with 300μl of Wash Buffer for 1-2 minutes each time.
Step 13
Add 90μl of TMB Substrate to each well. Cover with a new plate sealer and incubate at 37°C in the dark for 15-25 minutes (do not exceed 30 minutes). Note: This incubation time is for reference only, the optimal reaction time should be determined by the end-user and can be shortened or extended according to the actual color change. The reaction may be terminated when a gradient is apparent in the standard wells.
Step 14
Add 50μl of Stop Solution to each well. The color in the wells should change from blue to yellow immediately. Note: If the color in the wells is green or the color change does not appear uniform, gently tap the plate to ensure thorough mixing.
Step 15
Determine the optical density (O.D. value) of each well immediately using a microplate reader set to 450 nm.
Standard Curve - Human Eph Receptor A1/EphA1 ELISA Kit (A2358) - Antibodies.comEnlarge Image
Figure 1: Standard Curve - Human Eph Receptor A1/EphA1 ELISA Kit (A2358)
Representative standard curve for Human Eph Receptor A1/EphA1 ELISA Kit (A2358).
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