Human CD117 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human CD117 in the samples. A monoclonal antibody specific for human CD117 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for CD117, and CD117 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CD117 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Platform

Microplate

Detection Type

Colorimetric

Sample Type

Cell culture supernatant, serum and plasma.

Sensitivity

2.16 pg/ml

Product Range

31.25 - 2000 pg/ml

Specificity

This kit can assay both natural and recombinant human CD117.

Reactivity

Human

Cross Reactivity

This assay has shown no significant cross-reactivity.

Recovery

Storage

Store at 2 - 8°C.

Synonyms

C Kit, c-Kit, c-Kit Ligand, Kit, Kit Ligand, KIT oncogene, KIT proto oncogene receptor tyrosine kinase, KIT_HUMAN, Mast cell growth factor receptor, Mast/stem cell growth factor receptor Kit, Mast/stem cell growth factor receptor precursor, MGF, p145 c-kit, PBT, Piebald trait protein, Proto oncogene c Kit, Proto oncogene tyrosine protein kinase Kit, Proto-oncogene c-Kit, SCF Receptor, SCFR, soluble KIT variant 1, Steel Factor Receptor, Stem cell factor receptor, tyrosine protein kinase Kit, Tyrosine-protein kinase Kit, v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog, v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.