Human CCL5 ELISA Kit (A1406)

Human CCL5 ELISA Kit

Sandwich (quantitative)

Human CCL5 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human RANTES in the samples. A monoclonal antibody specific for human RANTES has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for RANTES, and RANTES present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of RANTES bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

0.53 pg/ml

This kit can assay both natural and recombinant human RANTES.

Human

This assay has shown no significant cross-reactivity.

97% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

Beta chemokine RANTES, Beta chemokine RANTES precursor, C C motif chemokine 5, CCL 5, CCL5_HUMAN, Chemokine (C C motif) ligand 5, Chemokine CC Motif Ligand 5, D17S136E, EoCP, Eosinophil chemotactic cytokine, MGC17164, RANTES, RANTES(4-68), Regulated upon activation normally T expressed and presumably secreted, SCYA 5, SCYA5, SIS delta, SIS-delta, SISd, Small inducible cytokine A5, Small inducible cytokine A5 (RANTES), Small inducible cytokine subfamily A (Cys Cys) member 5, Small-inducible cytokine A5, T cell specific protein p288 , T cell specific RANTES protein, T cell-specific protein P228, T-cell-specific protein RANTES, TCP 228, TCP228