Human CCL3 ELISA Kit (A1404)

Human CCL3 ELISA Kit

Sandwich (quantitative)

Human CCL3 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human CCL3 in the samples. A monoclonal antibody specific for human CCL3 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for CCL3, and CCL3 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CCL3 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

31.25 - 2000 pg/ml

This kit can assay both natural and recombinant human CCL3.

Human

This assay has shown no significant cross-reactivity.

94% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

C-C motif chemokine 3, G0/G1 switch regulatory protein 19-1, G0S19-1, Macrophage inflammatory protein 1-alpha, MIP-1-alpha, MIP1A, PAT 464.1, SCYA3, SIS-beta, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein