Human CaV1.3 ELISA Kit (A5977)

Human CaV1.3 ELISA Kit

Human CaV1.3 ELISA Kit is a sandwich (quantitative) Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human CaV1.3 in tissue homogenates or other biological fluids.

Sandwich (quantitative)

Human CaV1.3 ELISA Kit employs the Sandwich Enzyme Immunoassay (ELISA) technique for the quantitative measurement of human CaV1.3 in human. An antibody specific for CaV1.3 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the CaV1.3 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-CaV1.3 Antibody, which binds the captured CaV1.3 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and Avidin conjugated to Horseradish Peroxidase (Avidin-HRP) is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of CaV1.3 captured in each well. The concentration of CaV1.3 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colourmetric

Colorimetric Microplate Reader

Tissue homogenates or other biological fluids.

0.121 ng/ml

0.312-20 ng/ml

Human

Intra-assay: CV < 10%, Inter-assay: CV < 12%

Information online is representative. Always refer to the Product Manual inside the kit.

Store at +4°C. Do not use past expiration date!

alpha-1 polypeptide, CAC1D_HUMAN, CACH3, CACN4, CACNA 1D, Cacna1d, CACNL1A2, Calcium channel, Calcium channel L type alpha 1 polypeptide isoform 2, Calcium channel neuroendocrine/brain type alpha 1 subunit, Calcium channel voltage dependent L type alpha 1, Calcium channel voltage dependent L type alpha 1D subunit, CCHL1A2, isoform 2, L type, PASNA, SANDD, Voltage dependent L type calcium channel subunit alpha 1D, Voltage gated calcium channel alpha 1 subunit, Voltage gated calcium channel alpha subunit Cav1.3, Voltage gated calcium channel subunit alpha Cav1.3, Voltage-dependent L-type calcium channel subunit alpha-1D, Voltage-gated calcium channel subunit alpha Cav1.3