Human Cathepsin B ELISA Kit (A528)

Human Cathepsin B ELISA Kit

Sandwich (quantitative)

This Cathepsin B enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-Cathepsin B Antibody. Standards or samples are then added to the microtire plate wells and Cathepsin B, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of Cathepsin B present in the sample, Anti-Cathepsin B Antibody (Biotin) is added to each well to "sandwich" the Cathepsin B immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Cathepsin B will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Microplate

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

< 5 pg/ml

156-10,000 pg/ml

Human

Store at 2-8°C for 4 months or at -20°C for 8 months.

This product is for research use only. It is not intended for diagnostic or therapeutic use.