Human Cardiotrophin 1 ELISA Kit (A527)

$655
Freight Charges
Lead Time
7-10 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Human Cardiotrophin 1 ELISA Kit
Assay Type
Sandwich (quantitative)
Principle of Assay
This Cardiotrophin 1 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-Cardiotrophin 1 Antibody. Standards or samples are then added to the microtire plate wells and Cardiotrophin 1, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of Cardiotrophin 1 present in the sample, Anti-Cardiotrophin 1 Antibody (Biotin) is added to each well to "sandwich" the Cardiotrophin 1 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Cardiotrophin 1 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Serum, body fluids, tissue lysates, and cell culture supernatants.
Sensitivity
< 10 pg/ml
Product Range
156-1000 pg/ml
Reactivity
Human
Recovery
Storage
Store at 2-8°C for 4 months or at -20°C for 8 months.
Synonyms
Cardiophin 1, Cardiophin1, Cardiotrophin I, Cardiotrophin-1, Cardiotrophin1, CardiotrophinI, CT 1, CT-1, CT1, CTF 1, Ctf1, CTF1_HUMAN
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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