This Cardiac Troponin I enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-Cardiac Troponin I Antibody. Standards or samples are then added to the microtire plate wells and Cardiac Troponin I, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of Cardiac Troponin I present in the sample, Anti-Cardiac Troponin I Antibody (HRP) is added to each well to "sandwich" the Cardiac Troponin I immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Cardiac Troponin I will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Platform

Microplate

Detection Type

Colorimetric

Sample Type

Serum, cell culture supernatants, and tissue homogenates.

Sensitivity

10 pg/ml

Product Range

312-20,000 pg/ml

Reactivity

Human

Recovery

Storage

Store at 4°C for 6 months or at -20°C for 12 months Store secondary antibody at 4°C.

Synonyms

cardiac muscle, cardiomyopathy, dilated 2A (autosomal recessive), Cardiomyopathy, familial hypertrophic, 7, included, CMD1FF, CMD2A, CMH7, cTnI, Familial hypertrophic cardiomyopathy 7, MGC116817, RCM1, Tn1, Tni, TNN I3, TNNC 1, TNNC1, TNNI3, TNNI3_HUMAN, Troponin I, Troponin I cardiac, Troponin I cardiac muscle, Troponin I cardiac muscle isoform, Troponin I type 3 cardiac, troponin I, cardiac 3, TroponinI, Ttroponin I type 3 (cardiac)

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.