Human Aspartate beta Hydroxylase ELISA Kit is a sandwich (quantitative) Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Aspartate beta Hydroxylase in serum, plasma, tissue homogenates or other biological fluids.

Assay Type

Sandwich (quantitative)

Principle of Assay

Human Aspartate beta Hydroxylase ELISA Kit employs the Sandwich Enzyme Immunoassay (ELISA) technique for the quantitative measurement of human Aspartate beta Hydroxylase in human. An antibody specific for Aspartate beta Hydroxylase has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Aspartate beta Hydroxylase present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Aspartate beta Hydroxylase Antibody, which binds the captured Aspartate beta Hydroxylase present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and Avidin conjugated to Horseradish Peroxidase (Avidin-HRP) is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Aspartate beta Hydroxylase captured in each well. The concentration of Aspartate beta Hydroxylase can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Platform

Pre-coated Microplate (12 x 8 well strips)

Detection Type

Colourmetric

Instrument

Colorimetric Microplate Reader

Sample Type

Serum, plasma, tissue homogenates or other biological fluids.

Sensitivity

0.057 ng/ml

Product Range

0.156-10 ng/ml

Assay Time

1 - 4.5 Hours

Reactivity

Human

Recovery

Sample Type	n	Range	Average
Serum	5	80% - 102%	91%
EDTA Plasma	5	81% - 100%	90%
Heparin Plasma	5	80% - 89%	84%

Linearity

Sample Type	1:2	1:4	1:8	1:16
Serum (n=5)	87-91%	87-107%	74-101%	92-97%
EDTA Plasma (n=5)	90-105%	84-101%	90-101%	79-108%
Heparin Plasma (n=5)	84-95%	92-105%	82-105%	89-91%

Precision

Intra-assay: CV < 10%, Inter-assay: CV < 12%

General Notes

Information online is representative. Always refer to the Product Manual inside the kit.

Storage

Store at +4°C. Do not use past expiration date!

Synonyms

A beta H J J, AAH, ASP beta hydroxylase, Aspartyl/asparaginyl beta hydroxylase, ASPH, ASPH_HUMAN, BAH, Cardiac junctin, CASQ2BP1, HAAH, Humbug, JCTN, junctin, Peptide aspartate beta dioxygenase

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.