Human Annexin A4 ELISA Kit (A5226)

Human Annexin A4 ELISA Kit

Sandwich (quantitative)

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Microplate

Colorimetric

6.9 pg/ml

18.75 - 1200 pg/ml

1 - 4.5 Hours

Human

35 beta calcimedin, 35-beta calcimedin, AIV, Annexin 4, Annexin IV, Annexin IV (placental anticoagulant protein II), Annexin IV placental anticoagulant protein II, Annexin-4, AnnexinA4, AnnexinIV, ANX 4, ANX A4, ANX4, ANXA 4, ANXA4, ANXA4 protein, ANXA4_HUMAN, Carbohydrate binding protein P33/P41, Carbohydrate-binding protein p33/p41, Chromobindin 4, Chromobindin-4, Chromobindin4, DKFZp686H02120, Endonexin I, EndonexinI, Lipocortin IV, LipocortinIV, MGC75105, OTTHUMP00000160052, OTTHUMP00000202207, P32.5, P33/41, PAP II, PAP-II, PAPII, PIG 28, PIG28, Placental anticoagulant protein II, PP4 X, PP4-X, PP4X, Proliferation inducing gene 28, Proliferation inducing protein 28, Protein II, Xanx-4, ZAP 36, ZAP36