Human Angiopoietin-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human ANG-1 in the samples. A monoclonal antibody specific for human ANG-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, and ANG-1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for ANG-1 is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of ANG-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
2.60 pg/ml
Product Range
31.25 - 2000 pg/ml
Specificity
This kit can assay both natural and recombinant human ANG-1.
Reactivity
Human
Cross Reactivity
This assay has shown no significant cross-reactivity.
