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Anti-HSPA2 Antibody [ARC1415] (A9952)

Western Blot - Anti-HSPA2 Antibody [ARC1415] (A9952) - Antibodies.com Western Blot - Anti-HSPA2 Antibody [ARC1415] (A9952) - Antibodies.com Immunofluorescence - Anti-HSPA2 Antibody [ARC1415] (A9952) - Antibodies.com Immunofluorescence - Anti-HSPA2 Antibody [ARC1415] (A9952) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC1415] antibody to HSPA2 for WB and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-HSPA2 Antibody [ARC1415]
Description
Rabbit monoclonal [ARC1415] antibody to HSPA2.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 488-637 of human HSPA2 (P54652).
Sequence
ILNVTAADKSTGKENKITITNDKGRLSKDDIDRMVQEAERYKSEDEANRDRVAAKNALESYTYNIKQTVEDEKLRGKISEQDKNKILDKCQEVINWLDRNQMAEKDEYEHKQKELERVCNPIISKLYQGGPGGGSGGGGSGASGGPTIEE
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1415
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
70 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
70kDa, Hcp70.2, Heat shock 70 kDa protein 2, Heat shock protein 2, Heat shock protein 70.2, Heat shock related 70 kDa protein 2, Heat shock-related 70 kDa protein 2, Heat-shock protein, 70-KD, 2, Heat-shock protein, 70-KD, 3, HSP70 2, HSP70 3, Hsp70-2, HSP70-3, HSP70.2, HSP70A2, HSP72, HSP72_HUMAN, Hspt70, Hst70, MGC58299, MGC7795, MGC93458, OTTHUMP00000180664, Testis-specific heat shock protein-related
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-HSPA2 Antibody [ARC1415] (A9952) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-HSPA2 Antibody [ARC1415] (A9952)
Western blot analysis of extracts of various cell lines, using Anti-HSPA2 Antibody [ARC1415] (A9952) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western Blot - Anti-HSPA2 Antibody [ARC1415] (A9952) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-HSPA2 Antibody [ARC1415] (A9952)
Western blot analysis of extracts of various cell lines, using Anti-HSPA2 Antibody [ARC1415] (A9952) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunofluorescence - Anti-HSPA2 Antibody [ARC1415] (A9952) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-HSPA2 Antibody [ARC1415] (A9952)
Immunofluorescence analysis of C6 cells using Anti-HSPA2 Antibody [ARC1415] (A9952) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-HSPA2 Antibody [ARC1415] (A9952) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-HSPA2 Antibody [ARC1415] (A9952)
Immunofluorescence analysis of U-2 OS cells using Anti-HSPA2 Antibody [ARC1415] (A9952) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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