Anti-HSP90 beta Antibody [H9010] (A305130)

Name: Anti-HSP90 beta Antibody [H9010]
Brand: Antibodies.com
SKU: A305130
Price: 520.0 USD
Availability: InStock

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$520

Mouse monoclonal [H9010] antibody to HSP90 beta for WB, IHC, ICC/IF, IP, ELISA and Antibody Microarray.

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$40 to United States

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5-8 Business Days

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Specifications

Name

Anti-HSP90 beta Antibody [H9010]

Description

Mouse monoclonal [H9010] antibody to HSP90 beta.

Specificity

This antibody detects HSP90 beta specifically in all reactive species - except in Chicken, where it detects both alpha and beta isoforms.

Applications

WB, IHC, ICC/IF, IP, ELISA, Antibody Microarray

Dilutions

WB: 1:2500, IHC: 1:100

Reactivity

Human, Mouse, Rat, Rabbit, Chicken, Canine, Fish, Shark, Hamster

Immunogen

Recombinant human HSP90 beta protein.

Host

Mouse

Clonality

Monoclonal

Clone ID

H9010

Isotype

IgG2a

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

90 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.2, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

90 kda heat shock protein beta HSP90 beta, D6S182, FLJ26984, Heat shock 84 kDa, Heat shock 90kD protein 1, beta, Heat shock 90kDa protein 1 beta, Heat shock protein 90 alpha family class B member 1, Heat shock protein 90 kDa, Heat shock protein 90kDa alpha (cytosolic) class B member 1, Heat shock protein 90kDa alpha family class B member 1, Heat shock protein beta, Heat shock protein HSP 90 beta, Heat shock protein HSP 90-beta, HS90B_HUMAN, HSP 84, HSP 90, HSP 90 b, HSP 90b, HSP84, hsp90ab1, HSP90B, HSPC2, HSPCB

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (8)

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Figure 1: Western Blot - Anti-HSP90 beta Antibody [H9010] (A305130)

Western blot analysis of human Lysates showing detection of Hsp90 protein using Anti-HSP90 beta Antibody [H9010] (A305130) at 1:1,000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2).

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Figure 2: Western Blot - Anti-HSP90 beta Antibody [H9010] (A305130)

Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of Hsp90 protein using Anti-HSP90 beta Antibody [H9010] (A305130) at 1:1,000. The secondary antibody used was HRP Goat Anti-Mouse.

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Figure 3: Immunohistochemistry - Anti-HSP90 beta Antibody [H9010] (A305130)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-HSP90 beta Antibody [H9010] (A305130) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Epidermis.

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Figure 4: Immunohistochemistry - Anti-HSP90 beta Antibody [H9010] (A305130)

Immunohistochemistry analysis of mouse inflamed colon, fixed in formalin. The Primary Antibody used was Anti-HSP90 beta Antibody [H9010] (A305130) at 1:10,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Mouse at 1:2000 for 1 hour at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..

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Figure 5: Immunohistochemistry - Anti-HSP90 beta Antibody [H9010] (A305130)

Immunohistochemistry analysis of human colon carcinoma, fixed in formalin. The Primary Antibody used was Anti-HSP90 beta Antibody [H9010] (A305130) at 1:10,000 for 12 hours at 4°C. The secondary antibody used was Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5,000 for 1 hour at room temperature. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..

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Figure 6: Western Blot - Anti-HSP90 beta Antibody [H9010] (A305130)

Western blot analysis of human cell lysates from various cell lines showing detection of Hsp90 protein using Anti-HSP90 beta Antibody [H9010] (A305130) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

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Figure 7: Immunohistochemistry - Anti-HSP90 beta Antibody [H9010] (A305130)

Immunohistochemistry analysis of human colon carcinoma, fixed in formalin. The Primary Antibody used was Anti-HSP90 beta Antibody [H9010] (A305130) at 1:10,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Mouse at 1:2000 for 1 hour at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..

Enlarge Image

Figure 8: Immunohistochemistry - Anti-HSP90 beta Antibody [H9010] (A305130)

Immunohistochemistry analysis of mouse inflamed colon, fixed in formalin. The Primary Antibody used was Anti-HSP90 beta Antibody [H9010] (A305130) at 1:10,000 for 12 hours at 4°C. The secondary antibody used was Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5,000 for 1 hour at room temperature. Localization: Inflammatory and epithelial mucosa. Magnification: 40x. Inflammatory and epithelial mucosa. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..

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Mouse monoclonal [H9010] antibody to HSP90 beta for WB, IHC, ICC/IF, IP, ELISA and Antibody Microarray.

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