Anti-HSP90 Antibody [D7A] (A305112)

Western blot analysis of rat cell lysates showing detection of Hsp90 protein using Anti-HSP90 Antibody [D7A] (A305112) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

Immunohistochemistry analysis of mouse inflamed colon, fixed in formalin. The Primary Antibody used was Anti-HSP90 Antibody [D7A] (A305112) at 1:100,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Mouse at 1:2000 for 1 hour at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..

Immunohistochemistry analysis of human colon carcinoma, fixed in formalin. The Primary Antibody used was Anti-HSP90 Antibody [D7A] (A305112) at 1:100,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Mouse at 1:2000 for 1 hour at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..