Anti-HSP90 Antibody [8D3] (A305128)

Name: Anti-HSP90 Antibody [8D3]
Brand: Antibodies.com
SKU: A305128
Price: 655.0 USD
Availability: InStock

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$655

Mouse monoclonal [8D3] antibody to HSP90 for IP and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

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Specifications

Name

Anti-HSP90 Antibody [8D3]

Description

Mouse monoclonal [8D3] antibody to HSP90.

Specificity

This antibody co-immunoprecipitates HSP90 complexes, including HSP70, Hop, Ah receptors, glucocorticoid receptors, heme-regulated eukaryotic initiation factor 2a (eIF-2a) kinase (HRI).

Applications

IP, ICC/IF

Dilutions

ICC/IF: 1:100, IP: 1:1,000

Reactivity

Human, Mouse, Rat, Rabbit

Immunogen

Human Ah receptor (Aryl hydrocarbon receptor).

Host

Mouse

Clonality

Monoclonal

Clone ID

8D3

Isotype

IgM

Conjugate

Unconjugated

Purification

PEG purification.

Concentration

1 mg/ml

Molecular Weight

90 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Storage

Store at 4°C. Do not freeze!

Synonyms

Heat shock 86 kDa, Heat shock protein 90kDa alpha cytosolic class A member 1, Heat shock protein 90kDa alpha cytosolic class B member 1, Heat shock protein HSP 90 alpha, Heat shock protein HSP 90 beta, Heat shock protein HSP 90-alpha, HS90A_HUMAN, HSP 84, HSP 86, Hsp 90, HSP86, HSP90A, HSP90AA1, HSP90AB1, HSP90B, HSPC1, HSPC2, HSPCAL1, HSPCAL4, Renal carcinoma antigen NY-REN-38

Isotype Controls

Mouse IgM [PRF-03] (A86761)

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Immunofluorescence - Anti-HSP90 Antibody [8D3] (A305128)

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSP90 Antibody [8D3] (A305128), at 1:100 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Mouse (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Melanosome. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 complex Antibody. (C) Composite.

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Figure 2: Immunoprecipitation - Anti-HSP90 Antibody [8D3] (A305128)

Immunoprecipitation analysis of rabbit reticulocyte lysate using Anti-HSP90 Antibody [8D3] (A305128) at 1:1,000. Hsp90 complex isolated by immunoprecipitation.

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Figure 3: Immunofluorescence - Anti-HSP90 Antibody [8D3] (A305128)

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSP90 Antibody [8D3] (A305128), at 1:100 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Mouse (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Melanosome. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 complex Antibody. (C) Composite.

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