Anti-HSP90 Antibody [4F3.E8] (A305126)

Name: Anti-HSP90 Antibody [4F3.E8]
Brand: Antibodies.com
SKU: A305126
Price: 520.0 USD
Availability: InStock

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$520

Mouse monoclonal [4F3.E8] antibody to HSP90 for WB, IHC, ICC/IF, IP and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-HSP90 Antibody [4F3.E8]

Description

Mouse monoclonal [4F3.E8] antibody to HSP90.

Specificity

This antibody detects both alpha and beta forms of HSP90 equally well.

Applications

WB, IHC, ICC/IF, IP, ELISA

Dilutions

WB: 1:2,000, IHC: 1:100, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat, Plant

Immunogen

Recombinant Human HSP90 purified from E.coli.

Host

Mouse

Clonality

Monoclonal

Clone ID

4F3.E8

Isotype

IgG2a

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~90 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.2, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Heat shock 86 kDa, Heat shock protein 90kDa alpha cytosolic class A member 1, Heat shock protein 90kDa alpha cytosolic class B member 1, Heat shock protein HSP 90 alpha, Heat shock protein HSP 90 beta, Heat shock protein HSP 90-alpha, HS90A_HUMAN, HSP 84, HSP 86, Hsp 90, HSP86, HSP90A, HSP90AA1, HSP90AB1, HSP90B, HSPC1, HSPC2, HSPCAL1, HSPCAL4, Renal carcinoma antigen NY-REN-38

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Immunohistochemistry - Anti-HSP90 Antibody [4F3.E8] (A305126)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-HSP90 Antibody [4F3.E8] (A305126) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature.

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Figure 2: Immunohistochemistry - Anti-HSP90 Antibody [4F3.E8] (A305126)

Immunohistochemistry analysis of mouse muscle tissue, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-HSP90 Antibody [4F3.E8] (A305126) at 1:1,000 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature.

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Figure 3: Western Blot - Anti-HSP90 Antibody [4F3.E8] (A305126)

Western blot analysis of rat tissue lysate showing detection of Hsp90 protein using Anti-HSP90 Antibody [4F3.E8] (A305126) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

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Figure 4: Immunohistochemistry - Anti-HSP90 Antibody [4F3.E8] (A305126)

Immunohistochemistry analysis of human colon carcinoma, fixed in formalin. The Primary Antibody used was Anti-HSP90 Antibody [4F3.E8] (A305126) at 1:100,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Mouse at 1:2000 for 1 hour at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature. Localization: Inflammatory cells. Magnification: 40x.