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Anti-HSP70 Antibody (A304824)

Immunohistochemistry - Anti-HSP70 Antibody (A304824) - Antibodies.com Immunocytochemistry/Immunofluorescence - Anti-HSP70 Antibody (A304824) - Antibodies.com Western Blot - Anti-HSP70 Antibody (A304824) - Antibodies.com Immunohistochemistry - Anti-HSP70 Antibody (A304824) - Antibodies.com Western Blot - Anti-HSP70 Antibody (A304824) - Antibodies.com Immunohistochemistry - Anti-HSP70 Antibody (A304824) - Antibodies.com Immunocytochemistry/Immunofluorescence - Anti-HSP70 Antibody (A304824) - Antibodies.com
$500
Product Datasheet

Rabbit polyclonal antibody to HSP70 for WB, IHC, ICC/IF, IP and ELISA.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-HSP70 Antibody
Description
Rabbit polyclonal antibody to HSP70.
Applications
WB, IHC, ICC/IF, IP, ELISA
Dilutions
WB: 1:1,000, IHC: 1:100, ICC/IF: 1:100, IP: 1:100
Reactivity
Human, Mouse, Rat, Beluga, Bovine, Canine, Fish, Carp, Guinea Pig, Hamster, Monkey, Porcine, Sheep, Coral, Plant, Shark, L. amazonensis
Cross Reactivity
This antibody may cross-react with HSC70 at lower dilutions.
Immunogen
Recombinant Full length Human HSP70 Protein.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Peptide affinity purification.
Concentration
1 mg/ml
Molecular Weight
~70 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
DnaK type molecular chaperone HSP70 1, Epididymis secretory protein Li 103, FLJ54303, FLJ54370, FLJ54392, FLJ54408, FLJ75127, Heat shock 70 kDa protein 1, Heat shock 70 kDa protein 1/2, Heat shock 70 kDa protein 1A/1B, Heat shock 70kDa protein 1A, Heat shock 70kDa protein 1B, Heat shock induced protein, HEL S 103, HSP70 1, HSP70 1/HSP70 2, HSP70 1B, HSP70 2, HSP70 iA , HSP70-1/HSP70-2, HSP70-1A, HSP70.1, HSP70.1/HSP70.2, HSP70I, HSP71_HUMAN, HSP72, HSPA1, HSPA1A, HSPA1B
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immunohistochemistry - Anti-HSP70 Antibody (A304824) - Antibodies.comEnlarge Image
Figure 1: Immunohistochemistry - Anti-HSP70 Antibody (A304824)
Immunohistochemistry analysis of mouse inflamed colon, fixed in formalin. The Primary Antibody used was Anti-HSP70 Antibody (A304824) at 1:1,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at room temperature. Counterstain: Methyl Green at 200uL for 2 min at room temperature.
Immunocytochemistry/Immunofluorescence - Anti-HSP70 Antibody (A304824) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-HSP70 Antibody (A304824)
Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSP70 Antibody (A304824), at 1:100 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Western Blot - Anti-HSP70 Antibody (A304824) - Antibodies.comEnlarge Image
Figure 3: Western Blot - Anti-HSP70 Antibody (A304824)
Western blot analysis of mouse Pam212 cells showing detection of HSP70 protein using Anti-HSP70 Antibody (A304824) at 1:1,000 for 2 hours at room temperature. Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Donkey Anti-Rabbit IgG: HRP for 1 hour at room temperature.
Immunohistochemistry - Anti-HSP70 Antibody (A304824) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-HSP70 Antibody (A304824)
Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution. The Primary Antibody used was Anti-HSP70 Antibody (A304824) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at room temperature. Localization: Cytoplasm.
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