This antibody is conformation-specific for membrane-bound HSP70. It binds to cell surface HSP70 without permeabilization of cell membrane in tumor cell lines. It does not bind to intracellular HSP70 at low concentrations.
Applications
WB, ICC/IF, Flow Cytometry, FACS
Dilutions
WB: 1:1,000, ICC/IF: 1:100, FACS: 1:250
Reactivity
Human, Mouse, Rat, Bovine, Porcine
Cross Reactivity
This antibody cross-reacts with HSC70.
Immunogen
Human native HSP70 protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
1H11
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein G purification.
Concentration
500 µg/ml
Molecular Weight
~70 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Fluorescence Activated Cell Sorting analysis of human Jurkat E6.1 cells using Anti-HSP70 Antibody [1H11] (A304712) at 20 µg/ml for 40 min at 4°C. Counterstain: Propidium Iodide nuclear stain at 2.5 µg/ml for 5 minutes at room temperature. Isotype Control: Anti-mouse FITC at 1:32 for 15 min at room temperature (blue line).
![FACS - Anti-HSP70 Antibody [1H11] (A304712) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304712_1.png?profile=product_top)
![Immunocytochemistry/Immunofluorescence - Anti-HSP70 Antibody [1H11] (A304712) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304712_2.png?profile=product_top)
![FACS - Anti-HSP70 Antibody [1H11] (A304712) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304712_1.png?profile=product_top_thumb)
![Immunocytochemistry/Immunofluorescence - Anti-HSP70 Antibody [1H11] (A304712) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304712_2.png?profile=product_top_thumb)
![FACS - Anti-HSP70 Antibody [1H11] (A304712) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304712_1.png?profile=product_image)
![Immunocytochemistry/Immunofluorescence - Anti-HSP70 Antibody [1H11] (A304712) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304712_2.png?profile=product_image)
![FACS - Anti-HSP70 Antibody [C92F3A-5] (A304773) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304773_1.png?profile=product_alternative)
![Western Blot - Anti-HSP70 Antibody [N27F3-4] (A305087) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305087_1.png?profile=product_alternative)
![Immunohistochemistry - Anti-HSP70 Antibody [BB70] (A305113) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305113_1.png?profile=product_alternative)
![Western Blot - Anti-HSP70 Antibody [3A3] (A305077) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305077_1.png?profile=product_alternative)
![Western Blot - Anti-HSP70 Antibody [5A5] (A305076) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305076_1.png?profile=product_alternative)
![Western Blot - Anti-HSP70 Antibody [2A4] (A305075) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305075_1.png?profile=product_alternative)
![Western Blot - Anti-HSP70 Antibody [1.86] (A304774) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304774_1.png?profile=product_alternative)
