Anti-HSF1 Antibody [10H4] (A305102)

Immunohistochemistry analysis of human breast carcinoma, fixed in 10% formalin solution for 20 hours at room temperature. The Primary Antibody used was Anti-HSF1 Antibody [10H4] (A305102) at 1:8000 for 40 min. The secondary antibody used was Dako labeled Polymer HRP Anti-rat IgG, DAB Chromogen (brown) (Dako Envision+ System) for 30 minutes at room temperature. Counterstain: Mayer's Hematoxylin (purple/blue) nuclear stain for 1 minute at room temperature. Localization: Nuclear. Magnification: 100X.

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSF1 Antibody [10H4] (A305102), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rat (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Western blot analysis of human A431 and HEK293 cell lysates showing detection of HSF1 protein using Anti-HSF1 Antibody [10H4] (A305102) at 1:1,000.

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSF1 Antibody [10H4] (A305102), at 1:100 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.