Anti-HSD3B1 Antibody (A16048)

Name: Anti-HSD3B1 Antibody
Brand: Antibodies.com
SKU: A16048
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to HSD3B1 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-HSD3B1 Antibody

Description

Rabbit polyclonal antibody to HSD3B1.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human HSD3B1 (NP_000853.1).

Sequence

MTGWSCLVTGAGGFLGQRIIRLLVKEKELKEIRVLDKAFGPELREEFSKLQNKTKLTVLEGDILDEPFLKRACQDVSVIIHTACIIDVFGVTHRESIMNVNVKGTQLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQNGHEEEPLENTWPAPYPHSKKLAEKAVLAANGWNLKNGGTLYTCALRPMYIYGEGSRFLSASINEALNNNGILSSVGKFSTVNPVYVGNVAWAHILALRALQDPKKAPSIRGQFYYISDDTPHQSYDNLNYTLSKEFGLRLDSRWSFPLSL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

42 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

3 beta and steroid delta isomerase 1, 3 beta and steroid hydroxy delta 5 steroid dehydrogenase, 3 beta HSD I, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3-beta-HSD I, 3-beta-hydroxy-5-ene steroid dehydrogenase, 3BETAHSD, 3BH, 3BHS1_HUMAN, 3BHSD, Delta-5-3-ketosteroid isomerase, HGNC:5217, HSD3B, HSDB3, HSDB3A, Hydroxy delta 5 steroid dehydrogenase, Hydroxy delta 5 steroid dehydrogenase 3 beta and steroid delta isomerase 1, Progesterone reductase, SDR11E1, Steroid Delta-isomerase, trophoblast antigen, Trophoblast antigen FDO161G

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-HSD3B1 Antibody (A16048)

Western blot analysis of extracts of various cell lines, using Anti-HSD3B1 Antibody (A16048) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunofluorescence - Anti-HSD3B1 Antibody (A16048)

Immunofluorescence analysis of rat adrenal gland using Anti-HSD3B1 Antibody (A16048) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-HSD3B1 Antibody (A16048)

Immunofluorescence analysis of human placenta using Anti-HSD3B1 Antibody (A16048) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-HSD3B1 Antibody (A16048)

Immunofluorescence analysis of mouse adrenal gland using Anti-HSD3B1 Antibody (A16048) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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