Anti-HPRT Antibody [ARC1300] (A305963)

Name: Anti-HPRT Antibody [ARC1300]
Brand: Antibodies.com
SKU: A305963
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1300] antibody to HPRT for WB.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Knockout Validated Antibody

Specifications

Name

Anti-HPRT Antibody [ARC1300]

Description

Rabbit monoclonal [ARC1300] antibody to HPRT.

Applications

Dilutions

WB: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 119-218 of human HPRT11 (P00492).

Sequence

GDDLSTLTGKNVLIVEDIIDTGKTMQTLLSLVRQYNPKMVKVASLLVKRTPRSVGYKPDFVGFEIPDKFVVGYALDYNEYFRDLNHVCVISETGKAKYKA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1300

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

25 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

HGPRT, HGPRTase, HPRT 1, HPRT1, HPRT_HUMAN, Hypoxanthine guanine phosphoribosyltransferase, Hypoxanthine phosphoribosyltransferase 1, Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome), Hypoxanthine-guanine phosphoribosyltransferase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-HPRT Antibody [ARC1300] (A305963)

Western blot analysis of extracts of various cell lines, using Anti-HPRT Antibody [ARC1300] (A305963) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-HPRT Antibody [ARC1300] (A305963)

Western blot analysis of extracts from wild type(WT) and HPRT1 knockout (KO) 293T cells, using Anti-HPRT Antibody [ARC1300] (A305963) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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